Cathepsin D Inhibitor Screening Kit (Fluorometric)

Catalog number
K148-100
Name
Cathepsin D Inhibitor Screening Kit (Fluorometric)
Size
100 assays
Price
591.00 EUR
Supplier

Details

Kit's description
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin D Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin D substrate sequence GKPILFFRLK(Dnp)-D-R-NH2) labeled with MCA. Cathepsin D will cleave the synthetic substrate to release the quenched fluorescent group MCA, which can then easily be measured using a fluorometer or fluorescence plate reader at Ex/Em = 328/460 nm. The relative efficacy of test inhibitors are compared to the positive control inhibitor, Pepstatin A (IC50 < 0.1 nM). The Cathepsin D assay is simple, straightforward, and can be adapted to 96-well plate assays and is suitable for high throughput screening (HTS).
Highlights
• Detection method: Fluorescence (Ex/Em = 328/460 nm) • Kit size: 100 assays • Applications: Assay kit to detect putative Cathepsin D inhibitors
Kit's benefits
• Simple one-step procedure; takes only 1 hours • Fast, robust and convenient • Cathepsin-D inhibitor screening assay is a fast and robust assay to screen potential inhibitors • Positive control inhibitor, Pepstatin A is included in the kit
Category
Apoptosis Assay Kit
Kit's other name
none
Contents
• CD Reaction Buffer • CD Substrate (1 mM) • Cathepsin D, human (lyophilized) • Pepstatin A (1mM in DMSO)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
6-12 months
Species reactivity
see datasheet
Applications
Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Samples tested
Purified Cathepsin D, Recombinant Cathepsin D
Gene
CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.
Description
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.