CaspSELECT™ Caspase-3 Immunoassay Kit (Fluorometric)

Activation of caspase-3 plays a key role in initiation of cellular events during apoptosis. The CaspSELECT™ Caspase-3 Immunoassay Kit provides an effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates. The assay utilizes caspase-3 polyclonal antibody to capture activated caspase-3 from cell lysates. Caspase substrate DEVD-AFC is then added and is cleaved proportionally to the amount of activated caspase-3 in the cell lysate. The cleavage generates free AFC which can be analyzed fluorometrically (Ex./Em. = 400 nm/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of caspase-3. Other known caspases and non-specific proteases are not detected.

• Detection method: Fluorescence (Ex/Em 370:425/ 490:525 nm) • Species reactivity: Mammalian • Kit size: 100 assays • Applications: Effective immunosorbent enzyme assay for specific, quantitative detection of caspase:3 activity in microtiter plates.

• Simple one-step procedure; takes only ~3 hours • Fast and convenient

Apoptosis Assay Kit

none

• Cell Lysis Buffer • Coating Buffer • Anti-Caspase-3 Antibody (20X) • Blocking Buffer • Incubation Buffer • DTT (1 M) • DEVD-AFC Substrate (1 mM) • Positive Control (rh-Caspase-3) • Microtiter Plate • Adhesive Plate Cover

-20°C

gel pack

6-12 months

see datasheet

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Cell and tissue lysates

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.