Cathepsin H Inhibitor Screening Kit (Fluorometric)

Cathepsin H (CTSH, EC 3.4.22.16), a lysosomal cysteine protease, is important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. BioVision’s Cathepsin H Inhibitor Screening Kit utilizes the ability of an active Cathepsin H to cleave a synthetic AFC-based peptide substrate to release free AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin H-specific inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of Cathepsin H.

• Detection method:Fluorescence (Ex/Em = 400/505 nm) • Applications: Screen/study/characterize potential inhibitors of Cathepsin H

• Convenient & Rapid • High-throughput adaptable assay to screen/study/characterize the potential inhibitors of Cathepsin H • Cathepsin H inhibitor is included in the kit

Apoptosis Assay Kit, Cathepsin H

CTSH Inhibitor Screening Kit (Fluorimetric)

• CTSH Assay Buffer • CTSH Reagent • Human Cathepsin H • CTSH Substrate • CTSH Inhibitor (1 mM)

-20°C

gel pack

12 months

see datasheet

Screen/study/characterize potential inhibitors of Cathepsin H

CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.

Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.