Cathepsin E Activity Assay Kit (Fluorometric)

Cathepsin E (CTSE, EC: 3.4.23.34) is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that is found in highest concentration on the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. BioVision’s Cathepsin E Activity Assay Kit utilizes the ability of an active Cathepsin E to cleave a synthetic MCA-based peptide substrate to release free MCA, which can be easily quantified using a fluorometer or fluorescence microplate reader. This assay kit is simple, rapid and can detect Cathepsin E activity as low as 1 mU in biological samples.

• Detection method: Fluorescence (Ex/Em = 320/420 nm) • Applications: Detection of Cathepsin E activity in tissue and cell lysates : Determine activity of purified Cathepsin E

• Rapid & convenient • Detects Cathepsin E activity as low as 1 mU in biological samples

Apoptosis Assay Kit, Cathepsin E

CTSE Activity Assay Kit (Fluorometric)

• CTSE Assay Buffer • CTSE Lysis Buffer • CTSE Substrate • Human Cathepsin E (Positive Control) • MCA Standard (1 mM)

-20°C

gel pack

12 months

see datasheet

• Detection of Cathepsin E activity in tissue and cell lysates • Determine activity of purified Cathepsin E

• Cell and tissue lysates • Purified Cathepsin E

Biovision supplies other types of Assays as 1.

CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.