Cathepsin E Inhibitor Screening Kit (Fluorometric)

Cathepsin E (CTSE, EC: 3.4.23.34) is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that is found in highest concentration on the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of the gastric cancers. BioVision’s Cathepsin E Inhibitor Screening Kit utilizes the ability of Cathepsin E to cleave a synthetic MCA-based peptide substrate to release free MCA, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin E-specific inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the MCA fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize the potential inhibitors of Cathepsin E.

• Detection method:Fluorescence (Ex/Em = 320/420 nm) • Applications: Screen/study/characterize potential inhibitors of Cathepsin E

• Convenient & Rapid • High-throughput adaptable assay to screen/study/characterize the potential inhibitors of Cathepsin E • Cathepsin E inhibitor is included in the kit

Apoptosis Assay Kit, Cathepsin E

CTSE Inhibitor Screening Kit (Fluorimetric)

• CTSE Assay Buffer • Human Cathepsin E • CTSE Substrate • CTSE Inhibitor (1 mM Pepstatin A in DMSO)

-20°C

gel pack

12 months

see datasheet

Screen/study/characterize potential inhibitors of Cathepsin E

CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.

Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.