Granzyme B Activity Fluorometric Assay Kit

Granzyme B (GZMB, EC number 3.4.21.79) also known as Granzyme-2 is a serine protease stored in the granules of activated cytotoxic T cells and NK cells. Upon target cell contact, Granzyme B is directionally exocytosed and with the assistance of perforin enters the target cell. With its unique substrate specificity (Granzyme B prefers an aspartic acid residue at the P1 site of its substrates), Granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell. In BioVision’s Granzyme B Activity Assay Kit, active human Granzyme B enzyme hydrolyzes the specific substrate to release the quench of fluorescent group, which can be detected fluorometrically at Ex/Em = 380/500 nm. This Assay Kit is rapid, reliable and sensitive and can detect Granzyme B activity in various biological samples.

• Detection method: Fluorescence ( Ex/Em = 380/500 nm ) • Applications: Measurement of Granzyme B activity in various biological samples or purified Granzyme B activity, Screening Granzyme B inhibitors, characterize/Study Granzyme B inhibitors.

• Simple and Sensitive • Rapid and convenient • Kit contains all necessary reagents for measuring Granzyme B Activity.

Granzyme B

Granzyme-2 Activity Assay Kit

• Granzyme B Assay Buffer • Granzyme B Substrate • Positive Control (Granzyme B Enzyme, human recombinant) • AFC Standard (1mM)

-20°C

gel pack

12 months

see datasheet

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Biovision supplies other types of Assays as 1.