Granzyme B Inhibitor Screening Kit (Fluorometric)

Granzyme B (GZMB, EC number 3.4.21.79) also known as Granzyme-2 is a serine protease stored in the granules of activated cytotoxic T cells and NK cells. Upon target cell contact, Granzyme B is directionally exocytosed and with the assistance of perforin enters the target cell. With its unique substrate specificity (Granzyme B prefers an aspartic acid residue at the P1 site of its substrates), Granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell. In BioVision’s Granzyme B Inhibitor Screening Kit, active human Granzyme B enzyme hydrolyzes the specific substrate to release the quench of fluorescent group, which can be detected fluorometrically at Ex/Em = 380/500 nm. In the presence of potent Granzyme B Inhibitor, the hydrolyzation of substrate will be impeded. The Kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput screening of Granzyme B inhibitors or characterize/study Granzyme B inhibitors. Inhibitor control, Ac-IEPD-CHO is included to compare the efficacy of test inhibitors.

• Detection method: Fluorescence ( Ex/Em = 380/500 nm ) • Applications: Screening Granzyme B inhibitors, characterize/Study Granzyme B inhibitors.

• Simple, sensitive and high-throughput • Rapid and convenient • Includes Inhibitor control, Ac-IEPD-CHO.

Granzyme B

Granzyme-2 Inhibitor Screening Kit

• Granzyme B Assay Buffer • Granzyme B Substrate • Granzyme B Enzyme (human recombinant) • Inhibitor Control (Ac-IEPD-CHO, 250 μM)

-20°C

gel pack

12 months

see datasheet

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Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.