CaspGLOWTM Fluorescein Active Caspase-9 Staining Kit

Activation of caspases plays a central role in apoptosis. The CaspGLOW™ Fluorescein Active Caspase-9 Staining Kit provides a convenient means for sensitive detection of activated caspase-9 in living cells. The assay utilizes the caspase-9 inhibitor LEHD-FMK conjugated to FITC (FITC-LEHD-FMK) as a fluorescent marker. FITC-LEHD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-9 in apoptotic cells.

• Detection method: Fluorescence microscopy, Flow cytometry and fluorescence plate reader (Ex. = 485 nm and Em. = 535 nm) • Species reactivity: Mammalian • Kit size: Convenient sizes (25 and 100 assays) • Applications: Sensitive detection of activated caspase:9 in living cells.

• Simple one-step procedure; takes only 1-2 hours • Fast and convenient • The FITC label allows for direct detection of the activated caspases in apoptotic cells

Apoptosis Assay Kit

none

• FITC-LEHD-FMK • Wash Buffer • Z-VAD-FMK

-20°C

gel pack

6-12 months

see datasheet

Sensitive detection of activated caspase:9 in living cells.

Live cells

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.