CaspGLOWTM Red Active Caspase Staining Kit

Activation of caspases plays a central role in apoptosis. The CaspGLOWTM Red Active Caspase Staining Kit provides a convenient means for detecting activated caspases in living cells. The assay utilizes a caspase family inhibitor VAD-FMK conjugated to sulfo-rhodamine (Red-VAD-FMK) as the fluorescent marker. Red-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspases in apoptotic cells.

• Detection method: Fluorescence microscopy, fluorescence plate reader (Ex/Em = 540/570 nm) and Flow cytometry • Species reactivity: Mammalian • Kit size: Convenient sizes (25 and 100 assays) • Applications: Detection of activated caspases in living cells.

• Simple one-step procedure; takes only 1-2 hours • Fast and convenient • The red fluorescence label allows for direct detection of activated caspases in apoptotic cells

Apoptosis Assay Kit

none

• Red-VAD-FMK • Wash Buffer • Z-VAD-FMK

-20°C

gel pack

6-12 months

see datasheet

Detection of activated caspases in living cells.

Live cells

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

red dye