CaspSCREENTM Flow Cytometric Apoptosis Detection Kit

Activation of members of caspase-family proteases plays a key role in apoptosis. The CaspSCREENTM Flow Cytometric Apoptosis Detection Kit provides a convenient means for detecting activation of caspases by flow cytometry in living cells. The assay is based on the cleavage of (aspartyl)2-Rhodamine 110 (D₂R), a reported substrate for members of caspase family proteases. The caspase substrate D₂R is non-fluorescent, however, upon cleavage of the substrate by cellular caspases, the released rhodamine 110 gives rise to fluorescence that can be measured at excitation of 488 nm and emission of 530 nm.

• Detection method: Flow cytometry, L:1 channel (Ex/Em = 488/530 nm). • Species reactivity: Mammalian • Kit size: Convenient sizes (25 and 100 assays) • Applications: Detection of activated caspases in living cells.

• Simple one-step procedure; takes only 20-30 minutes • Fast and convenient • D₂R is more cell-permeable than other fluorometric caspase substrates

Apoptosis Assay Kit

none

• D₂R Reagent • DTT (1 M) • D₂R Incubation Buffer

-20°C

gel pack

6-12 months

see datasheet

Detection of activated caspases in living cells.

Live cells

This 1 is suited for programmed cell-death studies.The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.

Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.