Annexin V-FITC Apoptosis Kit Plus

The Annexin V-FITC Apoptosis Detection Kit Plus is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily be detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need of fixation. The Annexin V-FITC Apoptosis Detection Kit Plus includes annexin V-FITC, SYTOX green dye, and binding buffer. The SYTOX green dye is impermeant to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids. After staining a cell population with annexin V-FITC and SYTOX Green dye in the provided binding buffer, apoptotic cells show green fluorescence, dead cells show a higher level of green fluorescence and live cells show little or no fluorescence. These populations can easily be distinguished using a flow cytometry with the 488 nm line of an argon-ion laser for excitation. Both annexin V-FITC and SYTOX Green dye emit green fluorescence that can be detected in the FL1 channel, freeing the other channels for the addition of other probes in multi-color labeling experiments.

• Detection method: Flow cytometry (Ex = 488 nm; Em = 530 nm). • Species reactivity: Mammalian • Kit size: Convenient sizes (25, 100 and 400 assays) • Applications: Annexin V:FITC kit includes Annexin V:FITC for detecting apoptosis and also propidium iodide (PI) for detecting necrosis.

• Simple one-step procedure; takes only 10 minutes • Fast and convenient • The Annexin VFITC Plus kit includes sytox green dye (instead of PI) which stains necrotic cells with a higher level of green fluorescence than FITC does

Apoptosis Assay Kit

none

• Annexin V-FITC • SYTOX Green Dye • Binding Buffer

+4°C

gel pack

12 months

see datasheet

Annexin V:FITC kit includes Annexin V:FITC for detecting apoptosis and also propidium iodide (PI) for detecting necrosis.

Live cells

This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Biovision has more cell death kits and antibodies.This 1 is suited for programmed cell-death studies.

This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.

Anti-FITC Antibody