Annexin V-Cy3 Apoptosis Kit Plus

The assay is based on the observation that soon after initiating apoptosis, cells translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily be detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need of fixation. The Annexin V-Cy3 Apoptosis Detection Kit Plus includes annexin V-Cy3, SYTOX green dye, and binding buffer. The SYTOX green dye is impermeant to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids. After staining a cell population with annexin V-Cy3 and SYTOX Green dye in the provided binding buffer, apoptotic cells show red fluorescence, dead cells show green fluorescence and live cells show little or no fluorescence. These populations can easily be distinguished by Fluorescence microscopy using FITC and rhodamine filters or by flow cytometry using the FL1 channel (Ex. 488 nm/Em. 530 nm) for SYTOX Green dye and FL2 channel for Annexin V-Cy3 (Ex. 543 nm/Em. 570 nm).

• Detection method: flow cytometry using FL1 channel for SYTOX Green dye (Ex = 488 nm; Em = 530 nm) and FL2 channel for Annexin V:Cy3 (Ex = 543 nm; Em = 570 nm). • Species reactivity: Mammalian • Kit size: Convenient sizes (25, 100 and 400 assays) • Applications: Detecting apoptosis in living cells by flow cytometry or fluorescence microscopy. Cy3 shows brighter red fluorescence.

• Simple one-step procedure; takes only 10 minutes • Fast and convenient • Apoptotic cells show red fluorescence, dead cells show green fluorescence and live cells show little or no fluorescence.

Apoptosis Assay Kit

none

• Annexin V-Cy3 • SYTOX Green Dye • Binding Buffer

+4°C

gel pack

12 months

see datasheet

Detecting apoptosis in living cells by flow cytometry or fluorescence microscopy. Cy3 shows brighter red fluorescence.

Live cells

This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Biovision has more cell death kits and antibodies.This 1 is suited for programmed cell-death studies.

Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Annexin V- Apoptosis Kit Plus can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Biovision antibody is well suited for fluorescent microscopy.

cy3 conjugation kit