Intracellular Hydrogen Peroxide Detection Kit (cell-based)

Hydrogen Peroxide (H2O2) is a reactive oxygen species and by-product of oxygen metabolism. H2O2 serves as a key regulator for a number of oxidative stress related pathways and is linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, and a number of neurodegenerative diseases. BioVision’s Intracellular Hydrogen Peroxide Detection kit uses a dye that reacts with intracellular hydrogen peroxide to produce an orange color and fluorescence, which is proportional to the concentration of intracellular hydrogen peroxide. This kit provides a simple, easy to perform, histochemical method for identification of intracellular H2O2 in the samples.

• Detection method: fluorescence microscope (Ex/Em = 543/545:750 nm) • Applications: Screening/studying/characterizing stimulators/inhibitors that affect intracellular levels of hydrogen peroxide.

• Simple procedure • Histochemical method for identification of intracellular H2O2 in the samples

Oxidative stress, Hydrogen Peroxide

none

• Assay Buffer • Staining Dye

-20°C

gel pack

12 months

see datasheet

Screening/studying/characterizing stimulators/inhibitors that affect intracellular levels of hydrogen peroxide.

Adherent cells

cell

For cells, cell lines and tissues in culture till half confluency.The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.