Nitric Oxide Synthase (NOS) Activity Assay Kit (Colorimetric)

Nitric oxide synthases (EC 1.14.13.39) (NOS) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. In presence of NADPH, FAD, FMN, (6R)-5,6,7,8-tetrahydrobiopterin, calmodulin and heme, NOS catalyzes a five-electron oxidation of the guanidino nitrogen of L-arginine with molecular oxygen to generate NO and L-citrulline. There are three isoforms of NOS: endothelial (eNOS), neuronal (nNOS), and inducible (iNOS). nNOS accounts for the production of NO in central nervous system, where NO participates in cell communication and information storage. eNOS produces NO in blood vessels and is involved in regulation of vascular function. In contrast to other isoforms, iNOS is expressed de novo under oxidative stress conditions and produces large amounts of NO as a part of body’s defense mechanism. BioVision’s Nitric Oxide Synthase Activity Assay Kit provides an accurate and convenient method to assay NOS activity in a variety of samples. In this assay, nitric oxide generated by NOS undergoes a series of reactions and reacts with Griess Reagent 1 and 2 to generate a colored product with a strong absorbance at 540 nm. The assay is simple, sensitive and high-throughput adaptable and can detect as low as 5 µU of NOS activity.

Ultrasensitive, Simple, HTP adaptable colorimetric assay kit for NOS activity measurement. Detection method: Colorimetry (Abs: 540 nm) Applications: Detection of NOS activity Sample Type: Purified recombinant protein, Tissue or cell extracts

Simple, HTP adaptable protocol Sensitivity as low as 5 µU of NOS activity Colorimetric detection

Oxidative stress, Metabolism

NOS activity assay kit

NOS Assay Buffer NOS Dilution Buffer NOS Substrate NOS Cofactor 1 NOS Cofactor 2 (25X) Nitrate Reductase NOS (Positive Control) Enhancer Nitrite Standard Griess Reagent 1 Griess Reagent 2

-80°C

dry ice

12 months

Mammalian samples

Detection of NOS activity

• Purified recombinant protein • Tissue or cell extracts

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.