HRV 3C Protease Activity Assay Kit (Colorimetric)

Human rhinovirus (HRV) infections are the most frequent causative agents of common cold and various other upper respiratory tract infections. Rhinoviruses are members of the picornavirus family, which have a positive-sense, single-stranded RNA genome that is translated into a single polyprotein precursor. In the case of HRVs, the viral polyprotein is mainly processed by the proteases (2A and 3C) to generate functional proteins and enzymes. BioVision’s HRV 3C Protease Activity assay kit utilizes the ability of a 3C Protease (derived from a HRV rhinovirus-14, EC: 3.4.22.28) to cleave a chromogenic peptide substrate to release a chromophore (pNA) which can be easily quantified using a microplate reader. This assay kit is simple, rapid and can detect HRV 3C Protease activity as low as 50 ng in samples and of purified proteins.

• Detection method: Absorbance (OD= 405 nm). • Applications: Detection of HRV 3C Protease activity in samples Determine activity of purified HRV 3C Protease

Simple, rapid assay. Can detect HRV 3C Protease activity as low as 50 ng in samples

Protease Activity

Precission protease Activity Assay kit, 3C Protease Activity Assay Kit

HRV 3C Protease Assay Buffer HRV 3C Protease (10 μg, Positive Control) HRV 3C Protease Substrate pNA Standard (0.1 M)

-20°C

gel pack

12 months

not specified

• Detection of HRV 3C Protease activity in samples • Determine activity of purified HRV 3C Protease

Purified proteins and cell samples.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.