HRV 3C Protease Inhibitor Screening Kit (Colorimetric)

Human rhinovirus (HRV) infections are the most frequent causative agents of common cold and various other upper respiratory tract infections. Rhinoviruses are members of the picornavirus family, which have a positive-sense, single-stranded RNA genome that is translated into a single polyprotein precursor. In the case of HRVs, the viral polyprotein is mainly processed by the proteases (2A and 3C) to generate functional proteins and enzymes. BioVision’s HRV 3C Protease Inhibitor Screening Kit utilizes the ability of a 3C Protease (derived from a HRV rhinovirus-14, EC: 3.4.22.28) to cleave a chromogenic peptide substrate to release a chromophore (pNA) which can be easily quantified using a microplate reader. In the presence of a HRV 3C Protease-specific inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the absorbance. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of HRV 3C Protease.

• Detection method: Absorbance (OD= 405 nm). • Applications: Screening/studying/characterizing inhibitors of HRV 3C Protease.

Simple, rapid assay. HTP adaptable.

Protease Activity

Precission protease Inhibitor Screening kit, 3C Protease Inhibitor Screening Kit

HRV 3C Protease Assay Buffer HRV 3C Protease HRV 3C Protease Substrate HRV 3C Protease Inhibitor (2 mM)

-20°C

gel pack

12 months

not specified

Screening/studying/characterizing inhibitors of HRV 3C Protease.

Small molecules

Colorimetric assays or detection use UV absorption or enzymatic color reaction.

Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.