Total Collagen Assay Kit (Colorimetric)

Collagen is the most abundant insoluble protein found in the extracellular matrix and connective tissues. It can be found in skin, tendons, bone, cartilage, muscle, vitreous humor and ligaments, among other tissues. There are more than sixteen - well characterized types of collagens, but types I, II and III collagen comprise more than 80% content in mammals. The triple-helical structure of collagen is quite unique: it consists of a repeating pattern of a basic trimer: Glycine-Proline-Hydroxyproline. In cells, collagens are secreted as procollagens and these chains are transported into the Endoplasmic Reticulum, where, numerous post-translational modifications lead to the formation of a triple helix with disulfide bonds. Excessive production of collagen is linked to pathological conditions including liver cirrhosis, lung fibrosis, and tumor growth. BioVision’s Collagen Assay Kit is a simple and sensitive assay to detect small amounts of collagens in a variety of samples. The assay is based on the acid hydrolysis of samples to form hydrolysates and Hydroxyproline. This released Hydroxyproline gets oxidized to form a reaction intermediate, which further in the reaction, forms a chromophore (Abs 560 nm). The assay is simple, sensitive and specific for collagen and can detect as low as 0.5 µg of collagen in a variety of samples such as tissue homogenates, biological fluids and purified proteins.

• Detection method: Absorbance (OD = 560 nm). • Sample Types: Mammalian tissues, protein/peptide hydrolysates, serum, Urine • Applications: Measurement of collagen in various sample types.

• Simple, fast and convenient assay • Can detect as low as 0.5 µg of collagen in a variety of samples

Metabolism

none

Oxidation Buffer Chloramine T Concentrate Perchloric Acid/Isopropanol Solution DMAB Concentrate (in DMSO) Collagen I Standard (2 mg/ml)

-20°C

gel pack

12 months

Mammalian tissues, protein/peptide hydrolysates, serum, urine

Measurement of collagen in various sample types.

• Mammalian tissues • Protein/peptide hydrolysates • Serum • Urine

total

Biovision supplies other types of Assays as 1.

Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )

Colorimetric assays or detection use UV absorption or enzymatic color reaction.