Hemoglobin Colorimetric Assay Kit

Hemoglobin (Hb) is an iron containing globular metalloprotein found primarily in red blood cells. It carries oxygen from the lungs to the rest of the body as oxyhemoglobin and then returns back to the lungs as deoxyhemoglobin. In most vertebrates, hemoglobin is in tetrameric form comprising two α-globin chains and two β-globin chains (α2β2). Each chain is associated with a non-protein heme group. Aside from oxygen transport, hemoglobin can bind and transport other molecules like nitric oxide and carbon monoxide. Hemoglobin also plays an important role in maintaining the shape of the red blood cells. Alterations in the structure and binding capacity of hemoglobin are associated with many blood disorders such as sickle-cell anemia and thalassemia. BioVision’s Hemoglobin Colorimetric Assay kit provides a quick and easy method for monitoring hemoglobin levels in a wide variety of samples. In this assay, the detector selectively converts heme into a stable chemical complex that absorbs maximally at 575 nm. The intensity of the color is directly proportional to the total concentration of hemoglobin in the sample. The kit can detect as low as 0.02 g/dl hemoglobin

• Detection method: Absorbance (OD = 575 nm) • Sample Types: Blood, serum, plasma • Applications: Estimation of Hemoglobin in various biological samples

• Simple one-step procedure; takes only 15 minutes • Fast and convenient • Detects physiological concentrations of hemoglobins in human samples • HTP adaptable

Metabolism

none

Hemoglobin Detector Hemoglobin Standard (equivalent to 1 g/dl)

+4°C

gel pack

12 months

Mammalian

Estimation of Hemoglobin in various Biological Samples

Blood, serum, plasma

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.