α-L-Fucosidase (FUCA1) Assay Kit (Colorimetric)

α-L-Fucosidase (EC 3.2.1.51) (FUCA1) is a hydrolase that is able to cleave α-L-fucosyl moieties from glycoconjugates and oligosaccharides. Fucosidase plays pivotal roles in cell differentiation, apoptosis, inflammation and host-pathogen interaction. Furthermore, abnormal concentrations of this glycosidase have been observed in patients suffering from cancer, fucosidosis, rheumatoid arthritis, cystic fibrosis, and leukocyte adhesion deficiency. Industrial applications of α-L-Fucosidase (FUCA1) include synthesis of fucosylated analogs that could serve as antiadhesion compounds, cancer vaccines, and anti-inflammatory therapeutics. BioVision’s α-L-Fucosidase Assay Kit provides a simple, sensitive and high-throughput adaptable approach to detect physiological concentrations of this glycosidase in a variety of biological samples. In this assay, α-L-Fucosidase uses a synthetic chloro p-nitrophenol derivative (R-pNP) as an α-L-Fucosidase substrate and releases a chlorinated pNP derivative which can be measured kinetically under acidic conditions (OD 405 nm). The assay involves a one-step simple reaction, with minimal sample preparation and does not need a stop solution to complete the reaction. The assay can detect as low as 20 µU of α-L-Fucosidase activity in a variety of samples.

• Detection method: Absorbance (OD = 405 nm). • Sample Types: Biological fluids such as serum, plasma, saliva, etc. • Applications: Estimation of FUCA1/AFU in various biological samples.

• Simple, fast and convenient assay • Can detect as low as 20 µU of α-L-Fucosidase activity in a variety of samples.

Apoptosis Assay Kit, Metabolism, Cancer

FUCA1 Assay kit

FUCA1 Assay Buffer FUCA1 Substrate pNP Standard (10 mM) DTT (1 M) FUCA1 Positive Control

-20°C

gel pack

12 months

Mammalian

Estimation of FUCA1/AFU in various biological samples.

Biological fluids such as serum, plasma, saliva, etc.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.