Angiotensin I Converting Enzyme Activity (ACE1) Assay Kit (Fluorometric)

Angiotensin I converting enzyme (ACE1, EC: 3.4.15.1), a dipeptidyl carboxypeptidase, is part of the renin-angiotensin system (RAS) that controls regulation of blood pressure by cleaving the C-terminal dipeptides of angiotensin I and bradykinin. It is found on the luminal surface of vascular endothelial cells, especially in pulmonary tissues. Elevated levels of ACE1 are found in patients suffering from sarcoidosis, leprosy, hyperthyroidism, acute hepatitis, primary biliary cirrhosis, diabetes mellitus, multiple myeloma, osteoarthritis, amyloidosis, Gaucher’s disease, pneumoconiosis, histoplasmosis and miliary tuberculosis. BioVision’s ACE1 Activity Assay Kit utilizes an active ACE1 to cleave a synthetic o-aminobenzoyl peptide (Abz-based peptide) substrate to release a fluorophore. The released Abz can be easily quantified using a fluorescence microplate reader. This assay kit is simple, rapid and can detect ACE activity as low as 10 mU in biological samples

• Detection method : Fluorescence (Ex/Em = 330/430 nm) • Applications: Detection of ACE1 activity in tissue/cell lysates

• Simple one-step reaction • Takes only 1-2 hrs • Non-radiometric fluorescent detection • HTP adaptable

Protease Activity

none

• ACE1 Assay Buffer • ACE1 Lysis Buffer • ACE1 Dilution Buffer • Positive Control • ACE1 Substrate • Abz-Standard (1 mM)

-20°C

gel pack

12 months

Mammalian

Detection of ACE1 activity in tissue/cell lysates

• Mammalian tissues: lung, heart, kidney • Serum, plasma

Biovision supplies other types of Assays as 1.

Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.