Lysosome Purification Kit

Lysosomes are membrane-bound cell organelles found in most animal cells (except red blood cells). Lysosomes are spherical vesicles containing hydrolytic enzymes capable of breaking down different kinds of biomolecules, including proteins, nucleic acids, carbohydrates, lipids, and cellular debris. They are known to contain over 50 different enzymes, which are active under acidic conditions. Thus, lysosomes act as the waste disposal system of the cells by digesting unwanted materials in the cytoplasm. BioVision’s Lysosome Isolation Kit provides a procedure for isolating enriched or purified lysosomal fraction from animal tissues and cultured cells by differential centrifugation followed by density gradient centrifugation

Applications: • Isolation of high purity lysosomes from tissues and cultured cells • Lysosome studies like enzyme activity or uptake studies • Protein profiling of lysosomes by SDS:PAGE and Western blot

• Simple & convenient protocol • The kit provides unique formulations of ready-to-use buffers and reagents to isolate lysosomes with minimal sample preparation

Cell Fractionation, Lysosome Isolation

none

• Lysosome Isolation Buffer • Lysosome Enrichment Buffer • Lysosome Gradient • Protease Inhibitor Cocktail

-20°C

gel pack

12 months

see datasheet

• Isolation of intact functional lysosomes from tissues and cultured cells • Lysosome studies like enzyme activity or uptake studies • Protein profiling of Lysosomes by SDS-PAGE and Western blot

• Mammalian glands and soft tissues such as liver, kidney, etc. • Cultured eukaryotic cell lines

lysosome

Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Biovision supplies purification kits and ultra pure reagents.