EZExtract™ Core Histone Isolation Kit

Histones are small alkaline proteins that interact with eukaryotic DNA to form the fundamental subunit of chromatin known as the nucleosome. The core histones H2A, H2B, H3 and H4 form the central spooling unit of the nucleosome; with two copies of each comprising a histone octamer that interacts with the negatively charged DNA. They enable an ordered compaction of the DNA, as well as spatiotemporal control of gene expression and genomic replication throughout mitosis. Post-translational modification of this core complex results in structural changes within the chromatin and epigenetic control of gene expression. BioVision’s EZExtract™ Core Histone Isolation Kit offers an optimized suite of reagents to rapidly extract the total core histones while preserving acetylation from tissues and cultured cells for biochemical and epigenetic analyses. One hundred standard extractions at a scale of 107 cells or 100 mg tissues can be performed with the reagents in this kit. Yield of the total histone proteins is approximately 0.4 mg per 107 cells or 100 mg of tissue

Applications: • Convenient and fast isolation of histones from mammalian tissues • Protein profiling, post:translational modification and epigenetic analyses • Protein profiling of histones by SDS:PAGE and Western blot

• Simple & convenient protocol • The kit provides unique formulations of ready-to-use buffers and reagents to isolate histones with minimal sample preparation

Cell Fractionation, Histone isolation

none

• Lysis Buffer • Extraction Reagent • Neutralizing Buffer • DTT (1 M)

-20°C

gel pack

12 months

see datasheet

• Isolation of intact functional core histones from tissues and cultured cells • Protein profiling, post-translational modification and epigenetic analyses • Protein profiling of Histones by SDS-PAGE and Western blot

• Mammalian glands and soft tissues such as liver, spleen, lungs etc. • Cultured eukaryotic cell lines