Lysozyme Activity Assay Kit (Fluorometric)

Lysozyme (EC 3.2.1.17), also known as muramidase or N-acetylmuramide glycanhydrolase, is a hydrolase acting on glycosidic bonds. It hydrolyzes the β-(1-4)-glucosidic linkage between N-acetyl-muraminic acid and N-acetyl-D-glucosamine residues present in the mucopolysaccharide cell wall. Lysozyme is ubiquitously found in a wide range of biological fluids such as tears, saliva and tissues. It exhibits antibacterial, antitumor and immune modulatory activities. Elevated concentrations of lysozyme in urine and serum have been reported in patients suffering leukemia, tuberculosis, megaloblastic anemias, acute bacterial infections, ulcerative colitis, severe renal insufficiency, pyelonephritis and nephritis. BioVision’s lysozyme activity assay kit utilizes the ability of lysozyme to cleave a synthetic substrate to release the free fluorophore which can be easily quantified (Ex/Em= 360/445 nm). This kit provides a simple, ultra-sensitive assay that can detect as low as 2 µU/ml of Lysozyme activity in a variety of biological samples.

• Detection method: Fluorescence (Ex/Em = 360/445 nm) • Application: Detection of Lysozyme Activity in variety of samples

• Rapid, and convenient • The assay can detect Lysozyme activity as low as 2 µU/ml in a variety of biological samples

Protease, Lysosomal Activity

none

• Lysozyme Assay Buffer • Lysozyme Stop Buffer • Lysozyme Substrate (in DMSO) • Lysozyme Positive Control (lyophilized) • 4-Methylumbelliferone Standard (5 mM)

-20°C

gel pack

12 months

see datasheet

Detection of Lysozyme Activity in variety of samples

• Purified protein • Cell and tissue lysate • Cellular Extract • Lysosome • Immunoprecipitated samples

Biovision supplies other types of Assays as 1.