Human Calpain 1 Inhibitor Screening Kit (Fluorometric)

Calpain 1 is calcium-dependent, non-lysosomal cysteine protease with low calcium requirement. It is expressed ubiquitously and in the brain, mainly localized to the cell body and dendrites of neurons and to a lesser extent in axons and glial cells. Hyperactivation of calpains is implicated in a number of pathologies associated with altered calcium homeostasis such as Alzheimer’s disease as well as secondary degeneration resulting from acute cellular stress following cerebral (neuronal) ischemia, traumatic brain injury and spinal cord injury. BioVision’s human calpain 1 inhibitor screening kit is based on the detection of cleavage of calpain substrate Ac-LLY-AFC. Ac-LLY-AFC possess very weak fluorescence; upon cleavage of the substrate by calpain 1, free AFC can be quantified fluorometrically (Ex/Em = 400/505 nm). In the presence of potent Calpain 1 inhibitor, the hydrolyzation of substrate will be impeded. The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput screening of Calpain 1 inhibitors. Inhibitor control (Calpain inhibitor 1, ALLN) is included to compare the efficacy of test inhibitors.

• Detection method: Fluorescence (Ex/Em= 400/505 nm). • Species reactivity: Mammalian • Applications: The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput screening of Calpain 1 inhibitors. Inhibitor control (Calpain inhibitor 1, ALLN) is included to compare the efficacy of test inhibitors.

• Simple procedure • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable

Calpain Assay

none

• Assay Buffer • Substrate • Calpain 1 Enzyme • Inhibitor Control (Calpain Inhibitor 1, ALLN) [1 μM]

-80°C

dry ice

12 months

see datasheet

Inquire

N/A

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.