Proteasome Activity Fluorometric Assay Kit

Proteasomes are very large (20S, 26S) protein assemblies found in both the nucleus and cytoplasm of all eucaryotes (and in some procaryotes) which are responsible for the degradation and recycling of proteins which have been previously tagged with ubiquitin. Such tagged proteins are degraded into peptides approximately 7-8 amino acids long which are subsequently further degraded. The 20S assembly is the functional protease structure with chymotrypsin-like, trypsin-like and caspase-like protease activities. BioVision’s Proteasome Activity Assay takes advantage of the chymotrypsin-like activity, utilizing an AMC-tagged peptide substrate which releases free, highly fluorescent AMC in the presence of proteolytic activity. The kit also includes a positive control (Jurkat Cell lysate with significant proteasome activity) and a specific proteasome inhibitor MG-132 which suppresses all proteolytic activity due to proteasomes, which make the assay specifically measures proteasome activity from other protease activity which may be present in samples.

• Detection method: Fluorescence (Ex/Em 350/440nm) • Species reactivity: Mammalian • Applications: Specifically measures proteasome activity from other protease activity present in samples.

• Simple one-step procedure; takes only 30-60 minutes • Accurate and easy to use

Metabolism

none

• Proteasome Assay Buffer • Proteasome Substrate (In DMSO) • Proteasome Inhibitor (In DMSO) • AMC Standard (1 mM in DMSO) • Positive Control

-20°C

gel pack

12 months

see datasheet

Inquire

Whole cell lysates, nuclear and cytoplasmic lysates

Biovision supplies other types of Assays as 1.