TMRE Mitochondrial Membrane Potential Assay Kit (Fluorometric)

Mitochondria are the powerhouse of the cells and generate energy in the form of ATP. Mitochondria utilize oxidizable substrates to produce a membrane potential in the form of a proton gradient across the mitochondrial inner membrane. Mitochondrial membrane potential is tightly interlinked to many mitochondrial processes, including ATP synthesis, ROS, calcium sequestration and import of proteins into the mitochondrion. Therefore, pharmacological changes in mitochondrial membrane potential are associated with several pathological parameters. BioVision’s TMRE Mitochondrial Membrane Potential Assay Kit uses TMRE (tetramethylrhodamine, ethyl ester) to label active mitochondria. TMRE is a cell-permeable, positively-charged, red-orange dye that readily accumulates in active mitochondria. Protonophores like FCCP and CCCP induces dissipation of mitochondrial membrane thus results in compromised staining.

• Detection method: Fluorescence (Ex/Em = 535/587 nm), FACS [FL:3 (Red) channel], Fluorescent microscopy [red filter (Ex/Em = 549/575 nm)] • Applications: Labeling of active mitochondria in cells : Screening of compounds which may compromise mitochondrial membrane potential

• Convenient method • Based on cell-permeable, positively-charged, red-orange dye TMRE, that readily accumulates in the active mitochondria • Includes Protonophores FCCP to validate the assay

Apoptosis Assay Kit, Mitochondrial membrane potential

Mitochondrial Membrane Potential Assay Kit (Fluorometric)

• Assay Buffer • TMRE Dye (1 mM, in DMSO) • Negative Control (FCCP, 40 mM)

-20°C

gel pack

12 months

see datasheet

• Labeling of active mitochondria in cells • Screening of compounds which may compromise mitochondrial membrane potential

• Adherent and suspension cells

mitochondrial

Biovision supplies other types of Assays as 1.

Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.