Mitochondrial Permeability Transition Pore Assay Kit

Mitochondria are the power centrals of the cell and play an essential role in energy production. Damage to mitochondria activates signaling pathways that induce apoptosis. The mitochondrial permeability transition pore (MPT pore or MPTP) is a nonspecific channel formed by components of the inner and outer mitochondrial membranes, and appears to be involved in the release of mitochondrial components during cell death. In healthy cells, MPTP’s flicker between open and closed states but during cell death MPTP’s dramatically alter the permeability of the mitochondria. Cytochrome c release and loss of mitochondrial membrane potential are subsequent to continuous pore activation. BioVision’s Mitochondrial Permeability Transition Pore Assay Kit provides a direct method of measuring cell death by measuring MPTP opening rather than relying on mitochondrial membrane potential alone.

• Detection method: Flow cytometry (Ex 488 nm) • Species reactivity: Mammalian • Applications: • Measure mitochondrial permeability transition pore activity in cells • Assessment of physiological mediators and antibodies that affect cell viability/cytotoxicity

• Simple procedure • Fast, convenient and sensitive • The assay provides a direct method of measuring mitochondrial permeability transition pore activation as an early marker of apoptosis

Apoptosis Assay Kit

none

• MPTP Staining Dye • CoCl2 • Ionomycin • MPTP Wash Buffer

-20°C

gel pack

12 months

Mammalian

• Measure mitochondrial permeability transition pore activity in cells • Assessment of physiological mediators and antibodies that affect cell viability/cytotoxicity

Cells (adherent and suspension)

mitochondrial

Biovision supplies other types of Assays as 1.