Dihydrofolate Reductase Activity Kit (Colorimetric)

Dihydrofolate Reductase (DHFR; 5,6,7,8-tetrahydrofolate NADP oxidoreductase; EC 1.5.1.3), is a ubiquitous enzyme that is present in all eukaryotic and prokaryotic cells. It catalyzes the reduction of dihydrofolate (FH2) to tetrahydrofolate (FH4) using NADPH as a cofactor. FH4 is essential for a number of enzymes that are necessary for the de novo synthesis of purines, thymidylic acid and some amino acids. Inactivation of the DHFR enzymatic activity causes reduction of the intracellular level of FH4, inhibition of RNA and DNA synthesis, and cell death. For this reason, DHFR has been a critically important enzyme as a molecular target in drug discovery. Biovision’s Dihydrofolate Reductase assay kit is based on the ability of DHFR to catalyze the oxidation of NADPH. The reaction progress is followed by monitoring the decrease in absorbance at 340 nm. Our assay has been optimized to be carried out in a 96- well plate. The assay is simple, sensitive and can detect as low as 4 mU/ml in a variety of samples.

• Detection method: Absorbance (OD= 340 nm) • Application: Detection of Dihydrofolate Reductase activity

• Rapid, sensitive and convenient • The kit measures total Activity of DHFR Activity with limit of quantification 4 mU/ml using recombinant DHFR

none

• DHFR Assay Buffer • DHFR Substrate • Dihydrofolate Reductase • NADPH

-80°C

dry ice

12 months

Mammalian

Detection of Dihydrofolate Reductase activity

Purified recombinant protein Cell and tissue lysate

Colorimetric assays or detection use UV absorption or enzymatic color reaction.