Mitochondrial Apoptosis Detection Fluorometric Kit, MitoCapture

Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis. The MitoCapture™ Apoptosis Detection Kit provides a simple, fluorescent-based method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial transmembrane potential. The kit utilizes MitoCapture™, a cationic dye that fluoresces differently in healthy vs apoptotic cells. In healthy cells, MitoCapture accumulates and aggregates in the mitocondria, giving off a bright red fluorescence. In apoptotic cells, MitoCapture cannot aggregate in the mitochondria due to the altered mitochondrial transmembrane potential, and thus it remains in the cytoplasm in its monomer form, fluorescing green. The fluorescent signals can be easily detected by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using FITC channel for green monomers (Ex/Em = 488/530+ 30 nm) and (optional) PI channel for red aggregates (Em = 488/590+ 42 nm).

• Detection method: Fluorescence microscopy and Flow cytometry: FITC (Ex/Em = 488/530 ± 30 nm); and (optional) PI (Em = 488/590 ± 42 nm) • Species reactivity: Mammalian • Kit size: Convenient sizes (25 and100 assays) • Applications: Disruption of mitochondrial transmembrane potential is one of the earliest intracellular events that occur upon induction of apoptosis. The MitoCapture Mitochondrial Apoptosis Detection Kit provides a simple and sensitive in vitro assay for detecting the mitochondrial changes in apoptosis. It is highly sensive and detects apoptosis in living cells.

• Simple one-step procedure; takes only 30 minutes • Fast and convenient

Apoptosis Assay Kit

none

• MitoCapture Reagent • Incubation Buffer

-20°C

gel pack

12 months

see datasheet

Disruption of mitochondrial transmembrane potential is one of the earliest intracellular events that occur upon induction of apoptosis. The MitoCapture Mitochondrial Apoptosis Detection Kit provides a simple and sensitive in vitro assay for detecting the mitochondrial changes in apoptosis. It is highly sensive and detects apoptosis in living cells.

Live cells

mitochondrial

This 1 is suited for programmed cell-death studies.The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.