Nitric Oxide Fluorometric Assay Kit

Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. Since NO is rapidly converted to nitrite (NO²ˉ) and nitrate (NOᶟˉ), the total concentration of nitrite and nitrate is used as a quantitative measure of NO production. BioVision’s Nitric Oxide Fluorometric Assay Kit provides an accurate and convenient measurement of total nitrate/nitrite concentration in a simple two-step process. In the first step nitrate is converted to nitrite by nitrate reductase. In the second step, nitrite reacts with the fluorescent probe DAN (2, 3 diaminonaphthalene). NaOH enhances the fluorescent yield. The fluorescent intensity is proportional to the total nitric oxide production. The kit has been tested with culture media, plasma, and tissue homogenates.

• Detection method: Fluorescence (Ex/Em 380/461 nm) • Species reactivity: Mammalian • Applications: Measurement of total nitrate/nitrite concentration

• Simple procedure; takes only 2-3 hours • Fast and convenient • The fluorometric kit provides a simple two-step process for quantitative measurement of total nitrate/nitrite productions. The assay can be easily done in a 96-well plate.

Metabolism

Nitrogen Oxide Fluorometric Assay Kit

• Assay Buffer • Enzyme Cofactor • Enhancer • Nitrate Reductase • Nitrate Standard • Nitrite Standard • DAN Probe • Sodium Hydroxide • Microtiter plate • Plate Cover

-20°C

gel pack

12 months

see datasheet

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Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.