DNA Damage Quantification Colorimetric Kit

Catalog number
K253-25
Name
DNA Damage Quantification Colorimetric Kit
Size
25 assays
Price
561.00 EUR
Supplier

Details

Kit's description
Apurinic/apyrimidinic (AP) sites are one of the major types of DNA lesions formed during the course of base excision and repair of oxidized, deaminated or alkylated bases. It has been estimated that about 2x105 base lesions are generated per cell per day. The level of AP sites in cells can be a good indicator of DNA lesion and repair against chemical damage and cell aging. The DNA Damage Quantification Kit utilizes the ARP (Aldehyde Reactive Probe) reagent that reacts specifically with an aldehyde group which is the open ring form of the AP sites. After treating DNA containing AP sites with ARP reagents, AP sites are tagged with biotin residues, which can be quantified using avidin-biotin assay followed by a colorimetric detection. The kit provides the necessary reagents for convenient determination of abasic sites in purified DNA sample in 96-well plate format.
Highlights
• Detection method: Absorbance (450 and 650 nm) • Species reactivity: Mammalian • Applications: Kit provides all necessary reagents for convenient determination of DNA damage (abasic sites) in sample DNA
Kit's benefits
• Kit provides all necessary reagents for 96-well plate format
Category
Apoptosis Assay Kit
Kit's other name
none
Contents
• ARP Solution • TE Buffer • Glycogen Solution • 0 ARP-DNA Standard • 40 ARP-DNA Standard • DNA Binding Solution • HRP-Streptavidin • 10X Wash Buffer • HRP Developer • 96-well Microplate (8 x 12 strips)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Kit provides all necessary reagents for convenient determination of DNA damage (abasic sites) in sample DNA
Samples tested
Purified damaged DNA
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.