Mitochondria/Cytosol Fractionation Kit

The Mitochondria/Cytosol Fractionation Kit provides unique formulations of reagents for effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells. The enriched mitochondrial and cytosolic fractions can be used for studying apoptotic and signal transduction pathways to detect translocation of factors interested between the two fractions by Western blotting, ELISA, or other assays. Procedures are simple and easy to perform, no ultracentrifugations and toxic chemicals are involved.

• Detection method: Western blotting, ELISA, or other assays. • Species reactivity: Mammalian • Kit size: 25, 100 assays • Applications: Effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells.

• Simple procedure; takes only 3-4 hours • Fast and convenient • The fractionation procedure is simple and staightforward. No ultracentrifugations are required. No toxic chemicals are involved.

Apoptosis Assay Kit

none

• Mitochondria Extraction Buffer • 5X Cytosol Extraction Buffer • DTT (1 M) • Protease Inhibitor Cocktail

-20°C

gel pack

12 months

see datasheet

Effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells.

Cells and tissues