Cytochrome c Releasing Apoptosis Assay Kit

Cytochrome c plays an important role in apoptosis. The protein is located in the space between the inner and outer mitochondrial membranes. An apoptotic stimulus triggers the release of cytochrome c from the mitochondria into cytosol where it binds to Apaf-1. The cytochrome c/Apaf-1 complex activates caspase-9, which then activates caspase-3 and other downstream caspases. BioVision’s Cytochrome c Releasing Apoptosis Assay Kit provides an effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides unique formulations of reagents to isolate a highly enriched mitochondria fraction from cytosol. The procedure is so simple and easy to perform, no ultracentrifugation is required and no toxic chemicals are involved. Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit.

• Detection method: Western blotting • Species reactivity: Mammalian • Applications: Effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides an easy:to:use procedure and reagents for separating a mitochondria:enriched fraction from cytosol.

• Simple procedure; takes ~3 hours • Fast and convenient • Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit. The procedure is simple and straightforward. No ultracentrifugation is required.

Apoptosis Assay Kit

none

• Mitochondria Extraction Buffer • 5X Cytosol Extraction Buffer • DTT (1 M) • 500X Protease Inhibitor Cocktail • Anti-Cytochrome c mouse mAb (0.2 mg/ml)

-20°C

gel pack

12 months

see datasheet

Effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides an easy:to:use procedure and reagents for separating a mitochondria:enriched fraction from cytosol.

Cells and tissues

This 1 is suited for programmed cell-death studies.

Biovision supplies other types of Assays as 1.