Yeast Mitochondria Isolation Kit

Mitochondria are the power house of the cells because they generate most of the supply of energy in the form of adenosine tri-phosphate (ATP). Mitochondria are double membrane organelles: an outer membrane and a folded inner membrane called cristae. Isolated mitochondria is a useful tool to study mitochondrial respiration, assembly of the respiratory complexes, apoptosis, mtDNA and mtRNA, and for protein profiling. BioVision’s Yeast Mitochondria Isolation kit will enable fast and easy purification of mitochondria from yeast cells, utilizing yeast cell wall lysis and homogenization. This Mitochondria Isolation Kit is tested for Pichia pastoris & Saccharomyces cerevisiae & can be used to isolate mitochondria from other yeast. Under fermentable media, the yield is ~150-200 µg of mitochondria & under non-fermentable media (e.g. Glycerol) ~200-250 µg of mitochondria from a culture of OD ~20.

Application • Isolation of highly pure intact mitochondria from yeast cells. • Mitochondrial respiration studies, assembly of the complexes, apoptosis, mtDNA and mtRNA, and for protein profiling. • Western blot and ELISA

• Simple & convenient protocol • The kit provides unique formulations of ready-to-use buffers and reagents to isolate intact mitochondria

Mitochondria

none

• Buffer A • Buffer B • 1 M DTT • Homogenization Buffer • Lysis Enzyme Mix • Storage Buffer • Protease Inhibitor Cocktail

-20°C

gel pack

12 months

see datasheet

Inquire

Yeast cells culture.