GST Fluorometric Activity Assay Kit

Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds. The GST Fluorometric Activity Assay Kit provides a simple, fluorescence-based in vitro assay for detecting the GST activity using fluorescence plate reader. The assay utilizes monochlorobimane (MCB), a dye that reacts with glutathione. The free form of MCB is almost nonfluorescent, whereas the dye fluoresces blue (ex/em = 380/461 nm) when reacts with glutathione. GST catalyzes the MCB-glutathione reactions and the fluorescence levels are proportionally to the amounts of GST presence in the reaction. Thus, the GST level in samples can be easily detected using a fluorometer or a 96-well fluorometric plate reader. The kit can detect GST activity in crude cell lysate or purified protein fraction, and also quantitate GST-tagged fusion protein. Detects < 0.5 mU.

• Detection method: Fluorescence (Ex/Em 380/461 nm) • Species reactivity: Mammalian • Applications: GST level in samples can be easily detected using a fluorometer or a 96:well fluorometric plate reader. The kit can detect GST activity in crude cell lysate or purified protein fraction, and also quantitate GST:tagged fusion protein. Detects < 0.5 mU.

• Simple procedure; takes only ~1-2 hours • Fast and convenient

Metabolism

none

• GST Assay Buffer • GST Sample Buffer • MCB Substrate • Glutathione (lyophilized) • GST Standard (20 mU/µl)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.