Nitric Oxide Colorimetric Assay Kit

Catalog number
K262-200
Name
Nitric Oxide Colorimetric Assay Kit
Size
200 assays
Price
469.00 EUR
Supplier

Details

Kit's description
Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. NO is rapidly oxidized to nitrite and nitrate which are used to quantitate NO production. BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process. The first step converts nitrate to nitrite utilizing nitrate reductase. The second step uses Griess Reagents to convert nitrite to a deep purple azo compound. The amount of the azochromophore accurately reflects nitric oxide amount in samples. The detection limit of the assay is approximately 0.1 nmole nitrite/well, or 1 µM.
Highlights
• Detection method: Absorbance (540 nm) • Species reactivity: Mammalian • Applications: BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite in a simple two:step process.
Kit's benefits
• Simple two-step procedure; takes only ~1-1.5 hours • Fast and convenient • BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite.
Category
Nitric Oxide Assay
Kit's other name
none
Contents
• Assay Buffer • Enzyme cofactor • Enhancer • Nitrate Reductase • Nitrate Standard • Nitrite Standard • Griess Reagent R1 • Griess Reagent R2 • Microtiter Plate • Plate Cover
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.