Hydrogen Peroxide Colorimetric/Fluorometric Assay Kit

Hydrogen Peroxide is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-κB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down’s syndrome and immune system diseases. BioVision’s Hydrogen Peroxide Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric and fluorometric assay for measuring H₂O₂ in biological samples. In the presence of Horse Radish Peroxidase (HRP), the OxiRed Probe reacts with H₂O₂ to produce product with color (λmax = 570 nm) and red-fluorescent (Ex/Em=535/587 nm). The kit can perform 200 reactions by fluorometric method or 100 reactions by colorimetric method. The detection limit can be as low as 2 pmol per assay (or 40 nM concentration) of H₂O₂ in the sensitive fluorometric assay.

• Detection method: Absorbance (570 nm)and fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Applications: The kit provides a highly sensitive, simple, direct and HTS:ready colorimetric assay for measuring H₂O₂ in biological samples.

• Simple procedure; takes less than ~1 hours • Fast and convenient • The kit can perform 200 reactions by fluorometric method or 100 reactions by colorimetric method. The detection limit can be as low as 2 pmol per assay (or 40 nM concentration) of H₂O₂ in the sensitive fluorometric assay.

Hydrogen Peroxide Assay

none

• H₂O₂ Assay Buffer • OxiRed™ Probe • Dimethylsulfoxide (DMSO, anhydrous) • HRP • H₂O₂ Standard (0.88M)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.