Cytosol/Particulate Separation Kit

The location and translocation of proteins, signaling molecules, and other small molecules inside cells regulate cell growth, differentiation and many other cellular functions. Separating cytosol and particulate fractions is an important step in studying subcellular localization of cellular components. However, traditional methods usually take hours to separate cytosol from particulates, so that some cellular components especially small molecules and metabolites are getting diffused or redistributed during the separation procedure. The new Cytosol/Particulate Rapid Separation Kit physically separate cytosol from particulate compartments rapidly through an oil layer and thus the two fractions would not contact or diffuse to each other. Using the method, contaminations can be avoided even for small molecules. Subcellular localization and analyses of factors interested can be performed accurately.

• Species reactivity: Mammalian • Applications: The Cytosol/Particulate Separation Kit provides an easy and convenient system for a rapid separation of cytosol and particulate fractions. Assaying cellular distributions of small moleculars or proteins, and other signal transduction pathways

• Simple procedure; takes less than 1 hour • Fast and convenient • The new Cytosol/Particulate Rapid Separation Kit physically separate cytosol from particulate compartments rapidly through an oil layer and thus the two fractions would not contact or diffuse to each other. Using the method, contaminations can be avoided even for small molecules.

Cytosol Isolation

none

• Cell Suspension Buffer • Cytosol Releasing Buffer • Oil Layer • Particulate Layer

-20°C

gel pack

12 months

see datasheet

Inquire

Cell cultures (adherent and suspension), fresh and frozen tissues