Membrane Protein Extraction Kit

The Membrane Protein Extraction Kit provides optimized buffers and reagents for effective extraction of membrane proteins from mammalian tissues and cells. Unlike other available procedures that can only extract the total cellular membrane proteins (combinations of plasma and organelle membrane proteins), BioVision’s kit was designed to not only extract the total cellular membrane proteins, but also purify the plasma membrane proteins specifically. The procedure offers consistent yield and high purity (over 90%). Membrane proteins prepared using the kit can be utilized in a variety of applications, such as Western blotting, 2-D gels, and enzyme analyses, etc. The entire procedure takes less than 1 hour.

• Species reactivity: Mammalian • Applications: Western blotting, 2:D gels, and enzyme analyses, etc. The entire procedure takes less than 1 hour.

• Simple procedure • Fast and convenient • The Membrance Protein Extraction Kit provides an easy and complete protocol for isolating integral membrance proteins efficiently from cultured mammalian cells.

Membrane Protein Extraction

none

• Homogenize Buffer • Upper Phase Solution • Lower Phase Solution • Protease Inhibitor Cocktail

-20°C

gel pack

12 months

see datasheet

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Cell cultures (adherent and suspension), fresh and frozen tissues

Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.