FractionPREP™ Cell Fractionation Kit

This FractionPREP™ Cell Fractionation system is designed to provide reproducible extraction of four subcellular protein fractions (Cytosol, nucleus, membrane/particulate, and cytoskeletal fractions) from a single mammalian sample. The method is fast and simple, needing only 2 hours and no ultracentrifugation involved. All four protein fractions obtained are suitable for many downstream applications such as 1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.

• Species reactivity: Mammalian • Applications: 1:D or 2:D gel, enzyme activity assays, gel shift assay, and Western blotting.

• Simple procedure; takes less than 1 hour • Fast and convenient • The kit is designed for serial sample preparation of four distinct protein fractions including cytosol/particulate/cytoskeleton/nuclear fractions, from one sample.

Cell Fractionation Kit

none

• Cytosol Extraction Buffer (CEB) • Membrane Extraction Buffer-A (MEB-A) • Membrane Extraction Buffer-B (MEB-B) • Nuclear Extraction Buffer (NEB) • DTT (1 M) • Protease Inhibitor Cocktail (lyophilized)

-20°C

gel pack

12 months

see datasheet

Inquire

Cell cultures (adherent and suspension), fresh and frozen tissues

cell

For cells, cell lines and tissues in culture till half confluency.