Immunoprecipitation (IP) Kit

Immunoprecipitation (IP) is widely used in research and development, by which a protein can be selectively purified from samples. BioVision’s immunoprecipitation kit provides optimized buffers for preparing cell/tissue extracts, antigen binding and washing steps. The protein A/G Sepharose beads provided in the kit has higher binding capacity with broader antibody isotype binding than traditional protein A or protein G resins. The kit can be used in variety of immunoprecipitation or Co-IP studies.

Application: • Immunoprecipitation (IP) and Co:IP • Functional study of Immunoprecipitated proteins/complexes • SDS:PAGE or western blot analysis of Immunoprecipitated proteins/complexes

• Provides optimized buffers for preparing cell/tissue extracts, antigen binding and washing steps • The protein A/G Sepharose beads provided in the kit has higher binding capacity with broader antibody isotype binding than traditional protein A or protein G resins • Validated for functional assays as well as analysis of IP/Co-IP proteins/complexes

Immunoprecipitation

none

• Non-Denaturing Lysis Buffer • RIPA Lysis Buffer • Protease Inhibitor Cocktail (lyophilized) • 10X Wash Buffer • Protein A/G Sepharose

-20°C

gel pack

12 months

see datasheet

Inquire

• Tissue or cell extracts • Biological samples