Mitochondria Isolation Kit For Tissue & Cultured Cells

Mitochondria are the powerhouse of the cells because they generate most of the supply of energy in the form of adenosine tri-phosphate (ATP). Mitochondria are double membrane organelles: an outer membrane and a folded inner membrane called cristae. Isolated mitochondria are the sample of choice to study mitochondrial respiration, assembly of the respiratory complexes, apoptosis, mtDNA and mtRNA, and mitochondrial protein profiling. The kit offers two options for the isolation of intact mitochondria. The first option is utilizing a reagent-based method allowing parallel processing of multiple samples. The second option uses traditional dounce homogenization, which provides better mitochondrial yield.

• Useful for mitochondrial respiration studies, assembly of the complexes, apoptosis, mtDNA and mtRNA, and protein profiling. • Western blot and ELISA.

Simple & rapid method to isolate highly pure, intact and functional mitochondria from tissues and cultured cells.

Mitochondria

none

• Mitochondria Isolation Buffer • Reagent A • Storage Buffer

-20°C

gel pack

24 months

Mammalian

• Useful for mitochondrial respiration studies, assembly of the complexes, apoptosis, mtDNA and mtRNA, and protein profiling. • Western blot and ELISA.

• Mammalian tissues. • Cultured Cells.

tissue

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