Yeast Nuclei Isolation Kit

To understand the nature of the replication and expression of the yeast genome, it is essential to have a method for the preparation of nuclei at different stages of growth. BioVision’s Yeast Nuclei Isolation kit enables fast and easy purification of nuclei from yeast cells, utilizing yeast cell wall lysis and homogenization.

Application: • DNA:Protein interaction, RNA:Protein interaction and Protein:Protein interaction studies • DNase I footprinting analysis, Enzymatic Assays and Pull:down assay • Western blot and ELISA

Simple, rapid and easy method to purify yeast nuclei.

Yeast nucleus

none

• Buffer A • Buffer B • 1 M DTT • Lysis Enzyme Mix • Buffer N • Protease Inhibitor Cocktail (Lyophilized)

-20°C

gel pack

12 months

see datasheet

• DNA-Protein interaction, RNA-Protein interaction and Protein-Protein interaction studies • DNase I footprinting analysis, Enzymatic Assays and Pull-down assay • Western blot and ELISA

Yeast cell culture