Cell Surface Protein Isolation Kit

Cell surface proteins play a major role in signal transduction, cell adhesion, transport and serve as diagnostic and pharmacological targets. BioVision’s Cell Surface Protein Isolation Kit provides a simple and efficient method for the isolation of cell surface proteins. In this method, cells are first labeled with Sulfo-NHS-SS-Biotin, an amine-reactive, thiol-cleavable, biotinylation reagent. Cells are subsequently lysed and the labeled cell surface proteins are isolated using Streptavidin beads. The bound proteins are then released from beads by incubating with DTT solution. Biotinylation reagent is cell-membrane-impermeable with an extended spacer arm to reduce steric hindrances associated with streptavidin binding. This convenient kit provides all the required components for optimal labeling and isolation of the cell surface proteins.

• Detection method: Western Blotting • Applications: Isolated cell surface proteins can be used for various downstream applications such as Western Blotting or other structural and functional studies

• Simple and efficient method for the isolation of cell surface proteins • Includes all required components for optimal labeling and isolation of the cell surface proteins

Protein Extraction & Purification, cell surface protein isolation

none

• Quenching Solution • Lysis Buffer • Wash Buffer • Streptavidin Beads • PBS Tablet • TBS Tablet • Sulfo-NHS-SS-Biotin • DTT

4°C

gel pack

12 months

see datasheet

Isolated cell surface proteins can be used for various downstream applications such as Western Blotting or other structural and functional studies

Adherent and suspension cells

cell

For cells, cell lines and tissues in culture till half confluency.