MTT Cell Proliferation Assay Kit (Colorimetric)

Quantification of the number of viable cells is an indispensable tool in cell biology research. BioVision’s MTT Cell Proliferation Assay Kit is a sensitive method for quantification of viable cells in proliferation and cytotoxicity assay. The method is based on the conversion of water soluble MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) compound to an insoluble formazan product. Viable cells with active metabolism convert MTT into formazan, however, dead cells lose this ability. Thus color formation serves as a useful and convenient marker of only the viable cells. The measured absorbance (590 nm) is proportional to the number of viable cells. This assay kit provides an easy-to-use, non-radioactive, and high-throughput method for cell proliferation, cell viability, chemotaxis, cytotoxicity, and apoptosis.

• Detection: Absorbance (490 nm) • Applications: : Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients : Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals : Assessment of physiological mediators that inhibit cell growth

• Non-radioactive • High-throughput • The entire assay can be performed directly in a 96-well plate and does not require washing/harvesting/or solubilization steps.

Cell Proliferation Assay, MTT

none

• MTT Reagent • MTT Solvent

-20°C

gel pack

12 months

see datasheet

• Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients. • Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. • Assessment of physiological mediators that inhibit cell growth.

• Adherent and suspension cells • Proliferating and non-proliferating cells

cell

Biovision supplies other types of Assays as 1.

For cells, cell lines and tissues in culture till half confluency.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.