Quick Cell Proliferation colorimetric Assay Kit

Catalog number
K301-500
Name
Quick Cell Proliferation colorimetric Assay Kit
Size
500 assays
Price
297.00 EUR
Supplier

Details

Kit's description
The Quick Cell Proliferation Assay Kit provides all reagents and detailed instructions for a fast and sensitive quantification of cell proliferation and viability. The assay is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. Expansion in the number of viable cells resulted in an increase in the activity of the mitochondrial dehydrogenases, which leads to the increase in the amount of formazan dye formed. The formazan dye produced by viable cells can be quantified by multi-well spectrophotometer (microtiter plate reader) by measuring the absorbance of the dye solution at 440 nm. The assay can be used for the measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds. The new method is so simple, requiring no washing, no harvesting, and no solubilization steps, and is faster and more sensitive than MTT, XTT, or MTS-based assays. The entire assay can be performed in a microtiter plate.
Highlights
• Detection: Absorbance (420:480 nm) • Species reactivity: Mammalian • Kit size: 500, 2500 • Applications: This fast and sensitive cell proliferation assay uses a water:soluble tetrazolium salt WST:1. The assay measures cell proliferation in response to growth factors/cytokines/mitogens/and nutrients etc.
Kit's benefits
• Simple one-step procedure; takes less than 4 hours • Fast and convenient • The method is so easy that it requires no washing/harvesting/or solubilization steps and is faster and more sensitive than MTT/XTT/or MTS-based assays. The entire assay can be performed directly in a 96-well plate.
Category
Cell Proliferation Assay
Kit's other name
none
Contents
• WST-1 Reagent (lyophilized) • Electro Coupling Solution (ECS)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Cell cultures (adherent and suspension)
Tissue
cell
Test
Biovision supplies other types of Assays as 1.
Description
For cells, cell lines and tissues in culture till half confluency.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.