Ready-to-use Cell Proliferation Colorimetric Reagent, WST-1

The ready-to-use cell proliferation reagent, WST-1 provides a simple and accurate method to measure cell proliferation, which is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. Expansion in the number of viable cells results in an increase in the activity of the mitochondrial dehydrogenases, which in turn leads to increase in the amount of formazan dye formed. The formazan dye produced by viable cells can be quantified by measuring the absorbance at 440 nm. This new method is non-radioactive, rapid and more sensitive than MTT, XTT, or MTS-based assays. The entire assay can be performed in the same microtiter plate and does not require extra steps like washing, harvesting and cell solubilization.

• Detection method: Absorbance 440 nm • Species reactivity: Mammalian • Applications: Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. Assessment of physiological mediators and antibodies that inhibit cell growth.

• Simple one-step procedure; takes less than 4 hours • Fast and convenient • The method is so easy that it requires no washing/harvesting/or solubilization steps and is faster and more sensitive than MTT/XTT/or MTS-based assays. The entire assay can be performed directly in a 96-well plate.

Cell Proliferation Assay

none

• WST-1 Reagent (in electron coupling solution)

-20°C

gel pack

12 months

see datasheet

Inquire

Cell cultures (adherent and suspension)

cell

For cells, cell lines and tissues in culture till half confluency.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.