EZViable™ Calcein AM Cell Viability Assay Kit (Fluorometric)

Quantification of number of viable cells is an indispensible tool in Cell Biology research. BioVision’s Calcein AM Cell Viability Assay Kit is a fluorometric method for extremely sensitive quantification of viable cells that can detect as low as 50 viable cells in less than 30 min. Calcein AM is a non-fluorescent, hydrophobic compound that easily penetrates intact and live cells. Hydrolysis of Calcein AM by intracellular esterase produces a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm and can be measured at Ex/Em = 485/530 nm. The measured fluorescence intensity is proportional to the number of viable cells. This assay kit provides an easy-to-use, non-radioactive, and high-throughput method for cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis.

• Detection method: Fluorescence (Ex/Em = 485/530 nm) • Applications: : Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients : Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals : Assessment of physiological mediators and antibodies that affect cell growth.

• Convenient & non-radioactive • High-throughput • Highly-sensitive: detect as low as 50 viable cells in less than 30 min. • Suitable for cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis

Cell viability assay

none

• Calcein AM • Calcein Dilution Buffer

-20°C

gel pack

12 months

see datasheet

• Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients • Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals • Assessment of physiological mediators and antibodies that affect cell growth.

• Adherent and suspension cells • Proliferating and non-proliferating cells

cell

Biovision supplies other types of Assays as 1.

For cells, cell lines and tissues in culture till half confluency.