PicoProbe™ LDH-Cytotoxicity Fluorometric Assay Kit

Lactate Dehydrogenase (LDH, EC 1.1.1.27) is a stable enzyme and is present in all cell types. Cell damage leads to release of LDH enzyme into the media and its activity is widely used as a marker for cytotoxicity. It reversibly converts lactate into pyruvate, with the concomitant interconversion of NADH and NAD+. BioVision’s PicoProbe™ LDH-Cytotoxicity Fluorometric Assay Kit provides a sensitive, quick, and easy way for detection of LDH released from damaged cells. In this assay, LDH converts lactate to pyruvate and NADH, which reduces a proprietary probe to an intensely fluorescent product (Ex/Em = 535/587 nm). The amount of fluorescence is directly proportional to the number of damaged cells. The assay is adaptable to high-throughput format and can be completed in less than 20 min. Sensitivity: ~ 100 cells.

• Detection method: Fluorescence (Ex/Em = 535/587 nm) • Applications: Measurement of released LDH in culture medium • Evaluation of cytotoxicity effect of test compounds

• Highly-Sensitive: Detects as less as 100 cells. • Rapid protocol: requires less than 20 min. • High-throughput adaptable.

LDH, lactate dehydrogenase, LDH Cytotoxicity Assay, LDH Cytotoxicity, cytotoxicity assay

PicoProbe™ Lactate Dehydrogenase-Cytotoxicity Fluorometric Assay Kit

• LDH Assay Buffer • LDH Substrate Mix • PicoProbeTM • Cell Lysis Solution • LDH Positive Control

-20°C

gel pack

12 months

see datasheet

• Measurement of released LDH in culture medium • Evaluation of cytotoxicity effect of test compounds

Adherent or Suspension Cells

Biovision supplies other types of Assays as 1.

Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit