Cell-Mediated Cytotoxicity Fluorometric Assay Kit (7-AAD/CFSE)

Cell-mediated cytotoxicity is an important phenomenon characterized by cytolysis of a compromised cell in the body by immune system. Activation of immune system leads to removal of target cells infected by pathogens or transformed cells/cancer cells. This process is mediated by antibody-dependent cell-mediated cytotoxicity (ADCC), complement-mediated cytotoxicity, or lymphocyte-mediated cytotoxicity. BioVision’s Cell-Mediated Cytotoxicity Assay Kit contains carboxyfluorosuccinimide ester (CFSE), a green fluorescent probe that labels live target cells and 7-aminoactinomycin D (7-AAD), a red fluorescent probe that labels late apoptotic and necrotic target cells killed in the cytotoxicity assay. This assay does not require cell lysis and provides a direct measurement of cytotoxicity instead of indirect indicators such as release of ATP or lactate dehydrogenase activity. The flow cytometry-based method provides robust data and enables multi-parametric analysis.

• Detection method: Fluorescence (FL1 channel and FL3 channel) • Applications: Measurement of cell cytotoxicity in response to drug or toxin treatment • Quantification of the cytotoxic effect of immune effector cells on target cells • Assessment of physiological mediators and antibodies that affect cell cytotoxicity

• Direct method to measure cell-mediated cytotoxicity. • Does not require cell lysis. • Provides multi-parametric analysis.

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• Cytotoxicity Assay Buffer • 7-AAD Staining Solution • CFSE Staining Solution

-20°C

gel pack

12 months

see datasheet

• Measurement of cell cytotoxicity in response to drug or toxin treatment • Quantification of the cytotoxic effect of immune effector cells on target cells • Assessment of physiological mediators and antibodies that affect cell cytotoxicity

Adherent and suspension cells

cell

Biovision supplies other types of Assays as 1.

For cells, cell lines and tissues in culture till half confluency.