Cell-Mediated Cytotoxicity Fluorometric Assay Kit (7-AAD/CFSE)

Catalog number
K315-100
Name
Cell-Mediated Cytotoxicity Fluorometric Assay Kit (7-AAD/CFSE)
Size
100 assays
Price
328.00 EUR
Supplier

Details

Kit's description
Cell-mediated cytotoxicity is an important phenomenon characterized by cytolysis of a compromised cell in the body by immune system. Activation of immune system leads to removal of target cells infected by pathogens or transformed cells/cancer cells. This process is mediated by antibody-dependent cell-mediated cytotoxicity (ADCC), complement-mediated cytotoxicity, or lymphocyte-mediated cytotoxicity. BioVision’s Cell-Mediated Cytotoxicity Assay Kit contains carboxyfluorosuccinimide ester (CFSE), a green fluorescent probe that labels live target cells and 7-aminoactinomycin D (7-AAD), a red fluorescent probe that labels late apoptotic and necrotic target cells killed in the cytotoxicity assay. This assay does not require cell lysis and provides a direct measurement of cytotoxicity instead of indirect indicators such as release of ATP or lactate dehydrogenase activity. The flow cytometry-based method provides robust data and enables multi-parametric analysis.
Highlights
• Detection method: Fluorescence (FL1 channel and FL3 channel) • Applications: Measurement of cell cytotoxicity in response to drug or toxin treatment • Quantification of the cytotoxic effect of immune effector cells on target cells • Assessment of physiological mediators and antibodies that affect cell cytotoxicity
Kit's benefits
• Direct method to measure cell-mediated cytotoxicity. • Does not require cell lysis. • Provides multi-parametric analysis.
Category
cytotoxicity assay, Cell-Mediated Cytotoxicity assay, cytotoxicity, 7-AAD/CFSE cell-mediated cytotoxicity assay, 7-AAD/CFSE cell mediated cytotoxicity assay
Kit's other name
none
Contents
• Cytotoxicity Assay Buffer • 7-AAD Staining Solution • CFSE Staining Solution
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
• Measurement of cell cytotoxicity in response to drug or toxin treatment • Quantification of the cytotoxic effect of immune effector cells on target cells • Assessment of physiological mediators and antibodies that affect cell cytotoxicity
Samples tested
Adherent and suspension cells
Tissue
cell
Test
Biovision supplies other types of Assays as 1.
Description
For cells, cell lines and tissues in culture till half confluency.